Academic and industrial partners
Précédent
Suivant
Workpackage 5 (WP5) is focused on addressing three complex diseases (SLE, RA, and Sclerodermia) through cutting-edge and multidisciplinary technologies, integrating insights from genetics, deep immunophenotyping, transcriptomic and epitranscriptomic analyses, large scale quantitative proteomics, and patient exposure to better stratify the patients.
Multiparametric flow cytometry on PBMCs.
B and T cell purifications for:
• RNAseq Total library
• sc RNA seq VDJ library
Analysis:
• Supervized
• Unsupervized
Single-cell profiling of the patient T and B cells.
Oligoclonality of the TCR and BCR repertoire.
Ag-specific B cell sorting for Immune profiling.
Identification of module of co-regulated genes.
Circulating Biomarkers : Large‐scale quantitative clinical proteomics of patient’s serum.
Multiplexed quantification of chemokines and cytokines.
Autoantigen characterization : Characterization of autoantigens bound to AID-derived serum (LES and Sclerodermia).
Epitranscriptomics : Multiplexed RNA-Modifications quantification.
Multi-omics genomic medicine sequencing :
Long Reads DNA
• SNV and SV analysis
• Methlylation profile
• Long Reads transcriptomics
• Differential expression analysis
• Transcript analysis
Integrated OMICS anaylsis for molecular diagnostic stratification and therapeutic prediction.
Patients’s exposure to:
• Air pollution (remote sensor)
• Water pollution
• Soil contaminants
• Biocontaminants (molds, pollen)
• Infections
• Green, gray, blue spaces
• Diet
• Meteorological variables
• Lifestyle (tobacco, alcohol)
• Occupation
• Socio-economic status
• Etc…
Identification of Biomarkers.
Clustering / Stratification of patients in each AID (RA, SLE, Sclerodermia).
Comparative signature, Identification of sub-population for treatment strategy, Define deregulated pathways and new therapeutic target , in-depth analysis of risk factors, prognostic markers and efficacy of therapeutic interventions, Development of new therapeutic strategies.
Les autres groupes de travail pour mener à bien ce projet.
Restoring immune homeostasis through cell therapy and in vivo cell reprogramming
Comprehensive and predictive models to evaluate drug response and safety
Pour rester informé.e
Formulaire et plan
Faire un don, un legs...
